1 kb ladder invitrogen pdf files

This product is available in quickload purple format enjoy the convenience of a readytoload format. The bands of the ladder each contain from 1 to 12 repeats of a 1018bp dna fragment. The product is supplied in a solution of 10 mm trishcl, ph 8. The 100 bp dna ladder contains 11 discrete dna fragments ranging in size from 100 bp to 3,000 bp. The 1636bp band contains 10% of the mass applied to the gel. Try the 1 kb plus dna ladder from neb, a single convenient ladder for 100 bp up to 10 kb 100 bp and 1 kb ladder in one. The 1 kb dna ladder is a unique combination of a number of proprietary plasmids digested with appropriate restriction enzymes and pcr products to.

It is suitable for sizing linear doublestranded dna fragments from 100 bp to 12 kb. This marker is ideal for the size determination of pcr products. Use the neb 1 kb ladder for size markers between 500 bp and 10 kb. This marker can be used with either agarose or polyacrylamide gels. Invitrogen 1 kb plus dna ladder nondistribution product as customer accommodation. Identification of the substancemixture and of the companyundertaking product identifier 1 kb dna ladder or ruler trade namebrand invitrogen1 kb plus dna ladder synonyms deoxyribonucleic acid, aqueous solution reach number mixture, not applicable. The dna ladder consists of proprietary plasmids which are digested to completion with appropriate restriction enzymes to yield 10 bands suitable for use as molecular weight standards for agarose gel electrophoresis. The 1 kb plus dna ladder is suitable for sizing linear doublestranded dna fragments from 100 bp to 12 kb.

Identification of the substancemixture and of the companyundertaking product identifier 1 kb dna ladder or ruler trade namebrand invitrogen 1 kb plus dna ladder synonyms deoxyribonucleic acid, aqueous solution reach number mixture, not applicable. Use the 1 kb dna extension ladder to size linear doublestranded dna fragments from 500 bp40 kb. Thermo scientific generuler 1 kb plus dna ladder is recommended for sizing and approximate quantification of a doublestranded dna in the range of 75 bp to 20,000 bp on agarose gels. The ladder is prepared from a plasmid containing repeats of a 1018 bp dna fragment. Arial office theme 1 % agarose gel dna electrophoresis 2% agarose gel dna electrophoresis 3% agarose gel dna electrophoresis overall comparison overall comparison comparison 5 mikrol of 1 kb dna ladder in 1 %,2% and 3% agarose gel electrophoresis comparison 5 mikrol of 100 bp dna ladder in 1 %,2% and 3% agarose gel. Making 1kb ladder working solution university of san diego. How can i prepare working solution with 1 kb dna ladder supplied by promega. This ladder has 12 evenly spaced bands ranging from 1kb to 12kb, quick orientation band at 1,650bp that forms distinct doublet with 2kb band, and seven bands of round sizes below 1kb. Invitrogen trackit 1 kb plus dna ladder is ready to use and designed for sizing and approximate quantification of doublestranded dna in the range of 100 bp to 15,000 bp. The ladder may be radioactively labeled by one of the. Powerful tool for estimating molecular weight of linear, doublestranded. If you are viewing this page as a nonregistered user, the prices displayed is list price. By the use of these products you accept the terms and conditions of all applicable limited use label licenses. Mark a scale on the horizontal, x axis which can contain these three sizes, plus the starting point of zero.

This ladder has 12 evenly spaced bands ranging from 1kb to 12kb, quick orientation band at 1,650 bp that forms distinct doublet with 2kb band, and seven bands of round sizes below 1kb. Invitrogen 1kb dna extension ladder 100ug nucleic acid. To view your gsa or va contract pricing, log in using your account number, or become a registered user by contacting one of our customer service teams. The ladder consists of bands that are generated from pcr and restriction enzyme digestion of double stranded dna. One kb plus dna ladder is composed of 20 highly purified, doublestranded dna bands spanning 100bp to 12,000bp. The quickload 1 kb extend dna ladder is not intended for precise quantification of dna mass but can be used for approximating the mass of dna in comparably intense samples of similar size. Construction of pbad24ompa for modulating ompa expression in escherichia coli to assess role in conjugation bernard c. The dna ladder consists of proprietary plasmids and dna molecules which are digested to completion with appropriate restriction enzymes to yield bands suitable for use as molecular weight standards for agarose gel electrophoresis. The dna ladder consists of 15 dna fragments and is provided with 6x tritrack dna loading dye. Load on the gel for gels with other lane widths, the components of the mixture should be scaled either up or down. Weve run a marker which gives us three bands at 20 kb kilobases, or thousand bases, 5. The 1 kb dna ladder, containing linear doublestranded dna fragments, is suitable for sizing doublestranded dna from 250 bp to 10,000 bp. The 1kb plus dna ladder is ready to use, ranges from 25000250 base pairs, has intensities at 3k and 1k bp and is premixed with loading buffer.

Quickload 1 kb extend dna ladder new england biolabs. Invitrogen 25 bp dna ladder 50ug nucleic acid molecular weight markers biochemicals and reagents english. Goldbios readytouse 1kb ladder serves as a clear dna standard with band fragments and greater band intensity for reference at the 3kb and. This ladder consists of bands containing 18 repeats of a 1018bp dna fragment. Invitrogen 25 bp dna ladder 50ug nucleic acid molecular. Goldbios readytouse 1kb ladder serves as a clear dna standard with band fragments and greater band intensity for reference at the 3kb and 1kb bands. The 1 kb and 3 kb bands contain more dna to provide internal orientation. How to dilute dna ladders andersen lab, 2016 invitrogen 1. Generuler 1 kb dna ladder thermo fisher scientific. The 1kb dna ladder has thirteen bluntended fragments with sizes ranging from 250bp to 10,000bp. Material safety data sheet full scale dna ladder, 1kb. This products resides on a fisher scientific gsa or va contract.

Twofold serial dilutions of 1 kb plus dna ladder invitrogen were loaded in the amounts of 200 ng, 100 ng, 50 ng and 25 ng from left to right. Invitrogen 1 kb plus dna ladder 10787026 print share invitrogen 1 kb plus dna ladder. Comparison between 1 kb plus dna ladder and 1 kb dna ladder. The concentration of 1kb dna ladder is 100 ugml 500ul five microlitres 500 ng of the 1 kb dna ladder are mixed.

Quickload 1 kb dna ladder is a premixed, readytoload molecular weight marker containing bromophenol blue as a tracking dye. The trackit 1 kb dna ladder is supplied in a readytoload format. Thermo scientific generuler 1 kb dna ladder is designed for sizing and approximate. This ladder has 12 evenly spaced bands ranging from 1kb to 12kb, quick orientation band at 1,650bp that forms distinct doublet with 2kb band, and. In addition to these 12 bands, the ladder contains vector dna fragments that. This marker is ideal for the size determination of digested dna. Not intended for any animal or human therapeutic or diagnostic use. Thermo scientific generuler 1 kb dna ladder is recommended for sizing and approximate quantification of a doublestranded dna in the range of 250 bp to. The digested dna includes fragments ranging from 0.

The 1 kb dna ladder is a unique combination of a number of proprietary plasmids digested with appropriate restriction enzymes and pcr products to yield fragments, suitable for use as molecular weight standards for electrophoresis. Invitrogen 1 kb plus dna ladder is designed for sizing and approximate quantification of doublestranded dna in the range of 100 bp to 15000 bp. Generuler 1 kb plus dna ladder, readytouse thermo fisher. Readytouse 1 kb dna ladder 1 kb dna ladder is suitable for sizing linear doublestranded dna fragments from 250 bp to 10 kb. This ladder can be stained with ethidium bromide or radiolabeled using t4 dna polymerase or t4 polynucleotide kinase. Highranger plus 100bp dna ladder 100bp 0bp analyzing dna over a wide range of sizes. Use this product as directed in the protocol of choice. The dna 1 kb ladder is suitable for size determination of doublestranded dna using dna electrophoresis. In addition to these 12 bands, the ladder contains vector dna fragments that range from 75 to 1636 bp.

Contents amount storage sm0311 generuler 1 kb dna ladder 250 5 x 50 g for 500 applications, 0. Available on gsava contract for federal government customers. Use the invitrogen 1 kb plus dna ladder if you need size markers for fragments between 100 bp and 12 kb. The 125bp band is approximately 2 to 3 times brighter than the other bands, except for the 500bp and 2652bp bands. How to dilute dna ladders andersen lab, 2016 we currently have two different kinds of ladder in the lab. Ultraranger 1kb dna ladder 300bp 24000bp size determination of digested dna with high molecular weight more 100bp and 1 kb ladders are offered from our partner biovision. For easy reference on agarose gels, the 5kb band is two to threetimes brighter than the other bands in the ladder. If samples need to be diluted, use te or other buffer of minimal ionic strength. We currently have two different kinds of ladder in the lab. Thermo scientific generuler 1 kb dna ladder is designed for sizing and approximate quantification of wide. Generuler 1 kb dna ladder catalog number sm0311, sm0312 pub. The quickload 1 kb extend dna ladder is a premixed, readytoload molecular weight dna marker containing bromophenol blue dye as a tracking dye.

Comes supplied with 1 vial of gel loading dye, purple 6x, no sds. Exonuclease reaction degradation of dna from both 3. Tridye 1 kb dna ladder is a premixed, readytoload molecular weight marker containing 3 dyes which serve as visual aids to monitor the progress of migration during agarose gel electrophoresis. All yellow highlighted numbers need to line up with the corresponding bands in the gel slice. The 1kb dna extension ladder consists of 8 fragments ranging in length from 1,018 to 8,144bp in 1,018bp increments, as well as bands of 506bp, 517bp, 1,636bp, 10kb, 15kb, 20kb, and 40kb. Egel 1 kb plus dna ladder thermo fisher scientific. Supernatants of escherichia coli k12 wildtype strain and. Invitrogen 1 kb plus dna ladder is designed for sizing and approximate quantification of doublestranded dna in the range of 100 bp to 15,000 bp. A ready to use version with image green is also available from them. How can i prepare working solution with 1 kb dna ladder. View more information about our dna stains technology. Lo department of microbiology and immunology, ubc ompa is an abundant outer membrane protein in escherichia coli that has been shown to play an important role in bacterial conjugation by maintaining membrane stability.

For descriptions of symbols on product labels or product documents, go to. Two readytouse dna size markers for gel electrophoresis, the 100 bp ladder and 1 kb ladder represent the two most popular markers used for dna fragment size determination. Trackit 1 kb plus dna ladder thermo fisher scientific. The ladder consists of nine bands in even increments 0. If run on a 2% agarose gel, an additional band at 253 bp may be visible. Shop online for a wide selection of invitrogen 1 kb plus dna ladder powerful tool for estimating molecular weight of linear, doublestranded dna fragments. Sigmaaldrich offers a number of directload 1 kb dna ladder products. A number of proprietary plasmids are digested to completion with appropriate restriction enzymes to yield 10 bands suitable for use as molecular weight standards for agarose gel electrophoresis. This ladder consists of bands containing 1 8 repeats of a 1018bp dna fragment. The 1 kb dna ladder contains discrete dna fragments ranging in size from 300 bp to 10,000 bp.